Mutation Analysis Of Rs7903146 Transcription Factor 7 Like 2 (Tcf7l2) Gene In Type II Diabetes Mellitus Sufferers Using The PCR-RFLP Method

Abstract

Diabetes mellitus (DM) is a series of metabolic abnormalities characterized by increased blood glucose levels (hyperglycemia), which is caused by impaired insulin release, insulin function. The TCF7L2 gene is a gene associated with susceptibility to type 2 diabetes mellitus (T2D). Mutations in the TCF7L2 SNP rs7903146 gene, showed a significant correlation with T2D. The purpose of this study was to analyze the rs7903146 ​​mutation of the TCF7L2 gene in T2D patients in the Sidoarjo area using the PCR RFLP method. This study uses a descriptive research type with a qualitative approach. This research was conducted at the Molecular Biology Laboratory, Faculty of Health Sciences, Muhammadiyah University of Sidoarjo, in July-August 2024. The sampling technique used in this study was non-probability sampling, especially the purposive sampling technique at the Bhayangkara Hospital Pusdik Sabhara Porong for 1 month. The method used for analysis is the PCR RFLP method. This study used 18 samples. In this study, there were no mutations.