The Detection of Toxoplasma gondii in Cat Feces Using The Microscopic and Loop-Mediated Isothermal Amplification (LAMP) Method at The Larangan Market Sidoarjo

Abstract

Toxoplasmosis is a zoonotic disease caused by Toxoplasma gondii, which has a significant impact on humans and animals, especially individuals with weakened immune systems. This study aimed to detect T. gondii in cat feces using the saturated NaCl microscopy method, and Loop-Mediated Isothermal Amplification (LAMP) method, as well as to the LAMP method and to evaluated the sensitivity of the LAMP method. Sampling was conducted using the accidental sampling technique over one month in August. The study employed a descriptive method by comparing result obtained through the microscopy and LAMP methods, analyzed using the SPSS version 23 kappa test. The detection result showed 4 positive samples and 5 negative samples out of 9 cat fecal samples from Larangan Market Sidoarjo, tested through NaCl flotation and DNA isolation using the resin method, followed by LAMP reaction with color change indicators. Sensitivity testing was performed by diluting the samples five times PBS to optimize DNA quality. The analysis showed perceft agreement between the two methods (k = 1.000) with (p < 0,05), indicating high significance in detecting T.gondii. Future research is recommended to expand the number and types of cat feces samples form various market locations to echance population representation and the validity of the research findings.